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755168 · SolisFast Probe qPCR Mix 5X (with Rox) 5 x 1 ml

Variant(en)
• Mix for qPCR with SolisFast DNA polymerase and SolisGreen fluorescent DNA marker
• Ready to use: addition of matrix, primers and water only
• The 5X buffer contains: 7.5 mM of MgCl₂, dNTPs stabilizers and enhancers that maximize efficiency of PCR reaction
• Ready to use: addition of matrix, primers, probes and water only
• The 5X buffer contains: 15 mM of MgCl₂, dNTPs stabilizers and enhancers that maximize efficiency of PCR reaction
• Possibility of multiplex qPCRs (up to 5 simultaneous targets)
• Ready to use: addition of matrix, primers, probes and water only
• The qPCR 5X buffer contains: 16,5 mM MgCl₂ (1x PCR solution 3,3 mM MgCl₂), dNTPs (dATP, dCTP, dGTP, dUTP) and additives to ensure maximum efficiency of the PCR reaction
• The mix includes Uracil-DNA glycosylase (UNG)enzyme and dUTPs enzyme instead of dTTPs
• Reduces the risk of cross-contamination by eliminating DNA from previous PCR reaction:
- the uracil-DNA glycosylase (UNG) enzyme degrades the DNA containing uracil residues at the beginning of the reaction, before being inactivated during the activation phase of the polymerase
- the dUTPs are incoporated instead of dTTPs during the DNA elongation step
• Possibility of multiplex qPCRs (up to 5 simultaneous targets)




